Tissue Culture of the Enteric Nervous System from Equine Ileum

被引：0
作者：
N.P.H. Hudson
G.T. Pearson
I.G. Mayhew
机构：
[1] University of Edinburgh,Departments of Preclinical Veterinary Sciences and Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies
[2] University of Edinburgh,Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies
[3] University of Edinburgh,Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies
来源：
Veterinary Research Communications | 2000年 / 24卷
关键词：
culture; enteric nervous system; glia; horse; ileum; immunohistochemistry; neuron; submucous plexus;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Ileal samples were harvested fresh from euthanized adult horses. The tissues were microdissected to prepare wholemount preparations for immunohistochemistry and for either explant or dissociated culture systems of the enteric nervous system. Explant culture systems were established using wholemounts of either the submucous plexus or the muscularis externa (including the myenteric plexus). Dissociated cell cultures could only be obtained from the submucous plexus. Culture systems were maintained for up to 5 days. Immunoreactivity for a neuronal marker (Pan-N) and for glial cell markers (GFAP and S100) indicated the presence of both neurons and enteric glia in the tissue culture preparations.
引用
收藏
页码：299 / 307
页数：8
相关论文
共 76 条
  • [1] Bannerman P.G.C.(1987)Analysis of enteric neurons, glia and their interactions using explant cultures of the myenteric plexus Developmental Neuroscience 9 201-227
  • [2] Mirsky R.(1988)Establishment and properties of separate cultures of enteric neurons and enteric glia Brain Research 440 99-108
  • [3] Jessen K.R.(1991)Equine myenteric plexus with special reference to the pelvic flexure pacemaker The Anatomical Record 230 417-424
  • [4] Bannerman P.G.C.(1993)Neuropeptide distributions in the colon, cecum and jejunum of the horse The Anatomical Record 236 341-350
  • [5] Mirsky R.(1990)Equine myenteric ganglionitis: a case of chronic intestinal pseudo-obstruction Cornell Veterinarian 80 53-63
  • [6] Jessen K.R.(1984)Distribution of substance P-like immunoreactivity in the enteric neurons of the large colon of normal and amitraz-treated ponies: an immunocytochemical study Equine Veterinary Journal 17 23-29
  • [7] Burns G.A.(1991)Enteric glia Glia 4 195-204
  • [8] Cummings J.F.(1993)Neurons and glial cells of the enteric nervous system: studies in tissue culture Journal of Basic and Clinical Physiology and Pharmacology 4 157-179
  • [9] Burns G.A.(1999)An immunohistochemical study of interstitial cells of Cajal (ICC) in the equine gastrointestinal tract Research in Veterinary Science 66 265-271
  • [10] Cummings J.F.(1982)Ileocolonic aganglionosis in white progeny of overo spotted horses Journal of the American Veterinary Medical Association 180 289-292
12345678