Hemato-immunological and plasma biochemical responses of silvery-black porgy (Sparidentex hasta) fed protein and essential amino acid deficient diets

被引：2
作者：
Mozanzadeh M.T. [1 ]
Yaghoubi M. [1 ]
Marammazi J.G. [1 ]
Safari O. [2 ]
Gisbert E. [3 ]
机构：
[1] Agriculture Research, Education and Extension, Iran Fisheries Research Organization (IFRO), South Iran Aquaculture Research Center, Ahwaz
[2] Department of Fisheries, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi
[3] IRTA, Centre de Sant Carles de la Rápita (IRTA-SCR), Unitat de Cultius Experimentals, Crta. del Poble Nou Km 5.5, Sant Carles de la Rápita
来源：
Comparative Clinical Pathology | 2018年 / 27卷 / 1期
基金：
美国国家科学基金会;
关键词：
Digestive enzymes; Hematology; Immune responses; Liver; Plasma biochemistry; Sparidae;
D O I：
10.1007/s00580-017-2551-y
中图分类号：
学科分类号：
摘要：
A 6-week feeding trial was conducted to evaluate the effects of protein free (PF) and essential amino acid deficient (EAAD) diets on the physiological responses of silvery-black porgy (Sparidentex hasta) juveniles. Three experimental diets were formulated: a control diet in which 60% of dietary nitrogen was provided by intact protein (fish meal) and 40% by crystalline AA [(blends of essential amino acids (EAAs) and non-essential amino acids (NEAAs)]; an essential amino acid deficient diet in which 60% of dietary N was provided by intact protein, whereas the rest was provided by NEAAs; and a protein free (PF) diet, which based on carbohydrate sources. Fish fed the PF and EAAD showed signs of anemia including lower red blood cells counts, hemoglobin, and hematocrit levels than control group. Plasma lysozyme activity and complements C3 and C4, as well as total immunoglobulin levels were drastically reduced in fish fed PF and EAAD diets. Plasma and liver alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase were significantly increased, but superoxide dismutase was decreased in fish fed PF and EAAD diets. Plasma total protein, albumin, high density lipoprotein, calcium, and inorganic phosphorous significantly decreased in fish fed PF and EAAD diets. The information obtained from this study testing to extreme diets (EAAD and PF diets) may serve for better understanding the impact of protein nutritional imbalances in fish. © 2017, Springer-Verlag London Ltd.
引用
收藏
页码：55 / 60
页数：5
相关论文
共 33 条
[1]  
Abdel-Tawwab M., Ahmad M.H., Khattab Y.A., Shalaby A.M., Effect of dietary protein level, initial body weight, and their interaction on the growth, feed utilization, and physiological alterations of Nile tilapia, Oreochromis niloticus (L.), Aquaculture, 298, pp. 267-274, (2010)
[2]  
Barcellos L., Nicolaiewsky S., De Souza S., Lulhier F., Plasmatic levels of cortisol in the response to acute stress in Nile tilapia, Oreochromis niloticus (L.), previously exposed to chronic stress, Aquac Res, 30, pp. 437-444, (1999)
[3]  
Barcellos L.J.G., Kreutz L.C., Quevedo R.M., da Rosa J.G.S., Koakoski G., Centenaro L., Pottker E., Influence of color background and shelter availability on jundiá (Rhamdia quelen) stress response, Aquaculture, 288, pp. 51-56, (2009)
[4]  
Blaxhall P., Daisley K., Routine haematological methods for use with fish blood, J Fish Biol, 5, pp. 771-781, (1973)
[5]  
Booth M.A., Moses M.D., Allan G.L., Utilisation of carbohydrate by yellowtail kingfish Seriola lalandi, Aquaculture, 376-379, pp. 151-161, (2013)
[6]  
Calder P.C., Branched-chain amino acids and immunity, J Nutr, 136, pp. 288-293, (2006)
[7]  
Ellis A.E., Lysozyme assays, Techniques in fish immunology, pp. 101-103, (1990)
[8]  
Gao Y.J., Yang H.J., Liu Y.J., Chen S.J., Guo D.Q., Yu Y.Y., Tian L.X., Effects of graded levels of threonine on growth performance, biochemical parameters and intestine morphology of juvenile grass carp Ctenopharyngodon idella, Aquaculture, 424, pp. 113-119, (2014)
[9]  
Habte-Tsion H.-M., Liu B., Ge X., Xie J., Xu P., Ren M., Zhou Q., Pan L., Chen R., Effects of dietary protein level on growth performance, muscle composition, blood composition, and digestive enzyme activity of Wuchang bream (Megalobrama amblycephala) fry, Israel J Aquacul, 65, pp. 925-933, (2013)
[10]  
Holland M.C.H., Lambris J.D., The complement system in teleosts, Fish. Shellfish Immunol., 12, pp. 399-420, (2002)
1234