Gold nanoparticles induce DNA damage in the blood and liver of rats

被引：0
作者：
Eria Cardoso
Eduardo Londero
Gabriela Kozuchovski Ferreira
Gislaine Tezza Rezin
Elton Torres Zanoni
Frederico de Souza Notoya
Daniela Dimer Leffa
Adriani Paganini Damiani
Francine Daumann
Paula Rohr
Luciano da Silva
Vanessa M. Andrade
Marcos Marques da Silva Paula
机构：
[1] Universidade do Extremo Sul Catarinense,Laboratório de Síntese de Complexos Multifuncionais, PPGCS
[2] Instituto Federal de Educação,Laboratório de Biologia Celular e Molecular, PPGCS
[3] Ciência e Tecnologia Catarinense,Laboratório de Fisiopatologia Clínica e Experimental, PPGCS
[4] Campus Sombrio,undefined
[5] Universidade do Extremo Sul Catarinense,undefined
[6] Universidade do Sul de Santa Catarina,undefined
来源：
Journal of Nanoparticle Research | 2014年 / 16卷
关键词：
Gold nanoparticles; DNA damage; Comet assay; Blood; Liver;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The potential of gold nanoparticles (GNPs) for use in different biological applications has led to a strong interest in the study of their possible deleterious effects in biological systems and how these effects may be mitigated. This study was undertaken to investigate the effects of the acute and chronic administration of GNPs with mean diameters of 10 and 30 nm on deoxyribonucleic acid (DNA) damage in the blood and liver of adult rats. For the acute administration, Wistar adult rats received a single intraperitoneal injection of either GNPs or a saline solution. For the chronic administration, Wistar adult rats received a daily single injection of the same GNPs or saline solution for 28 days. Twenty-four hours after either the single (acute) or final injection (chronic), the rats were euthanised by decapitation, and the blood and liver were isolated for the evaluation of DNA damage. In this study, we demonstrated that the acute and chronic administration of GNPs 10 and 30 nm in size increased the frequency of DNA damage and the damage index in the blood and liver of adult rats. These findings suggest that the DNA damage may be caused by oxidative stress, which occurred regardless of the type of administration and GNP size.
引用
收藏
相关论文
共 227 条
  • [1] Alkilany AM(2010)Toxicity and cellular uptake of gold nanoparticles: what we have learned so far? J Nanoparticle 12 2313-2333
  • [2] Murphy CJ(2012)Green synthesis of gold nanoparticles using Spectrochimistry Acta 97 1-5
  • [3] Aromal SA(2009) foenum-graecum and its size-dependent catalytic activity Prog Brain Res 180 141-152
  • [4] Philip D(2009)Chapter 8 - nanoparticles: transport across the olfactory epithelium and application to the assessment of brain function in health and disease Nanotoxicology 3 161-171
  • [5] Aschner M(2001)CeO2 nanoparticles induce DNA damage towards human dermal fibroblasts in vitro Toxicol Appl Pharmacol 175 191-199
  • [6] Auffan M(1997)Size-dependent proinflammatory effects of ultrafine polystyrene particles: a role for surface area and oxidative stress in the enhanced activity of ultrafines Adv Cell Aging Gerontol 2 161-191
  • [7] Rose J(2005)Protein oxidation processes in aging brain Chem Commun 33 4181-4183
  • [8] Orsiere T(2007)Shape controlled growth of gold nanoparticles by a solution synthesis Nano Lett 7 1542-1550
  • [9] De Meo M(2014)Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes J Hazard Mater 264 303-312
  • [10] Thill A(2014)Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines Biochim Biophys Acta 1840 794-800
12345678910