Fumonisin B1 Affects the Biophysical Properties, Migration and Cytoskeletal Structure of Human Umbilical Vein Endothelial Cells

被引:0
作者
Xue Zhao
Yun Wang
Jiang-Li Liu
Jian-Hua Zhang
Shi-Chao Zhang
Yan Ouyang
Jiang-Tao Huang
Xiao-Yan Peng
Zhu Zeng
Zu-Quan Hu
机构
[1] Guizhou Medical University,Key Laboratory of Biology and Medical Engineering, Immune Cells and Antibody Engineering Research Center of Guizhou Province, School of Basic Medical Sciences/School of Biology and Engineering
[2] Guizhou Medical University,Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education
[3] Guizhou Provincial Center for Disease Control and Prevention,Institute of Public Health Testing and Evaluation
来源
Cell Biochemistry and Biophysics | 2020年 / 78卷
关键词
Fumonisin; Toxicity; Biophysical properties; Migration ability; Cytoskeletal structure; Human umbilical vein endothelial cells;
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学科分类号
摘要
Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial cells (HUVECs). Subsequently, changes in the cytoskeletal structure and its binding proteins were analyzed by immunofluorescence and real-time PCR, respectively. The results showed that FB1 could inhibit the viability of HUVECs in a dose-dependent manner. After treatment of HUVECs with FB1, the hypotonic resistance, cell surface charges, cell membrane fluidity, and migration ability were weakened, whereas the ROS levels were significantly increased. Moreover, the cytoskeletal structure of the HUVECs was significantly changed, and the mRNA expression of some important actin-binding proteins was altered. Therefore, this study revealed that FB1 can affect the migration and cytoskeletal structure of HUVECs, which provides a new perspective for further understanding the molecular mechanisms of FB1 toxicity.
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页码:375 / 382
页数:7
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