Visualization and quantification of protein-protein interactions in cells and tissues

Olink Bioscience has developed a protein detection assay that allows precise detection and quantification of proteins, protein interactions and modifications in fixed cells and tissue samples. Duolink® immunoassay reagents are based on in situ proximity ligation assays (PLA™), and the assay reports proteins or protein complexes as countable distinct fluorescent or chromogenic spots visualized by standard microscopy. This allows for robust validation of protein-interaction data to show an interaction in the correct cellular context at physiologically relevant expression levels.