High-resolution melting analysis: a promising molecular method for meat traceability

被引：0
作者：
Sasa Yang
Chunbao Li
Qiayu Wu
Changqing Zhu
Xinglian Xu
Guanghong Zhou
机构：
[1] Nanjing Agricultural University,Key Laboratory of Animal Products Processing, MOA, Key Laboratory of Meat Processing and Quality Control, MOE, Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology
来源：
European Food Research and Technology | 2014年 / 239卷
关键词：
DNA traceability; HRM; SNPs; Pork;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To find a promising molecular method for meat traceability, three methods of single nucleotide polymorphism (SNP) detection: RFLP-PCR analysis, high-resolution melting (HRM) analysis, and TaqMan probe analysis, have been compared in terms of accuracy, ease of use, throughput capability, and cost. We genotyped ten pork DNA samples across three SNPs. The results showed that the HRM genotyping method was the most accurate and easiest to use with the lowest cost, while TaqMan probe analysis provided similar results, but its cost was much higher.
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页码：473 / 480
页数：7
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