The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines

被引：0
|
作者：
Do Nyun Kim
Yoon-Jae Song
Suk Kyeong Lee
机构：
[1] Research Institute of Immunobiology,Department of Medical Lifescience
[2] College of Medicine,Department of Life Science
[3] The Catholic University of Korea,undefined
[4] Seoul 137-701,undefined
[5] Korea.,undefined
[6] Kyungwon University,undefined
[7] Seongnam 461-701,undefined
[8] Korea.,undefined
来源：
Experimental & Molecular Medicine | 2011年 / 43卷
关键词：
decitabine; DNA methylation; Herpesvirus 4, human; microRNAs; promoter regions, genetic;
D O I：
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摘要：
Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
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页码：401 / 410
页数：9
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