Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant

被引：0
作者：
Woro Triarsi Sulistyaningdyah
Jun Ogawa
Qing-Shan Li
Raku Shinkyo
Toshiyuki Sakaki
Kuniyo Inouye
Rolf D. Schmid
Sakayu Shimizu
机构：
[1] Kyoto University,Divisions of Applied Life Sciences
[2] Kyoto University,Food Science and Biotechnology, Graduate School of Agriculture
[3] Universität Stuttgart,Institut für Technische Biochemie
[4] Toyama Prefectural University,Biotechnology Research Center, Faculty of Engineering
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
bioremediation; cytochrome P450; dioxin; P450 BM-3; polychlorinated dibenzo-; -dioxin;
D O I：
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学科分类号：
摘要：
The metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 from Bacillus megaterium and a mutant enzyme of it (AL4V; Ala74Gly, Phe87Val, Leu188Gln triple mutant) was examined. Both purified enzymes metabolized 1-monochloro-, 2,3-dichloro-, and 2,3,7-trichloro-dibenzo-p-dioxin, but not 2,3,7,8-tetrachloro-dibenzo-p-dioxin. The mutant AL 4V had 2–12 times higher activity than the wild-type P450 BM-3 towards polychlorinated dibenzo-p-dioxins. The products were hydroxylated at an unsubstituted position and/or showing migration of the chloride and were less toxic derivatives with lower than 10% toxicity of the original compounds.
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页码：1857 / 1860
页数：3
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