Biogenic synthesis of copper nanoparticles using Vitis vinifera L. seed extract, and its in-vitro biological applications

The production of copper nanoparticles with the aqueous extract of dried grape seed is described in this paper. The UV–Vis spectra revealed absorption peaks above 350 nm, indicating that copper reduction resulted in nanoparticles production. The FTIR spectra of Cu nanoparticles revealed that they were surrounded by several organic compounds. The X-ray Diffractometer revealed intense peaks that corresponded to the face-centered cubic (fcc) structure's features. SEM confirmed that the produced nanoparticles ranged in size from 54 to 80.98 nm. EDS examination revealed the purity of CuNPs and only the existence of the copper phase in the samples. The nanoparticles' anti-diabetic efficacy was tested using the—amylase assay. The copper nanoparticle extract showed a percentage of inhibition of 32 percent at a concentration of 1000 μg/ml. CuNPs demonstrated a maximum zone inhibition of about 2 cm in E.coli, whereas the aqueous extract showed 1 cm, and a maximum zone inhibition of about 1.3 cm in Streptococcus Sp., whereas the aqueous extract showed 0.5 cm. The samples when treated on HCT-15 cell line, showed potent cytotoxicity from the MTT assay performed. Our research showed the eco- friendly method of synthesizing nanoparticle from seed extract which when compared to other synthesis methods proves to be a potential method.