Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL

被引:0
|
作者
Wen-Hsiu Hsu
Yih-Shou Hsieh
Hsing-Chun Kuo
Chun-Yuh Teng
Hai-I Huang
Chau-Jong Wang
Shun-Fa Yang
Yi-Sheng Liou
Wu-Hsien Kuo
机构
[1] Armed-Forces Taichung General Hospital,Division of Gastroenterology, Department of Internal Medicine
[2] Chung Shan Medical University,Institute of Biochemistry and Biotechnology, School of Medicine
[3] Central Taiwan University of Science and Technology,Department of Medical Technology
[4] Kaohsiung Medical Center,Department of Medical Research, Chang Gung Memorial Hospital
[5] National Defense Medical Center,Department of Public Health
[6] Armed-Forces Taichung General Hospital,Department of Medicine
来源
Archives of Toxicology | 2007年 / 81卷
关键词
Berberine; Colonic carcinoma; ROS; JNK; p38 MAPK;
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中图分类号
学科分类号
摘要
Berberine is the major constituent of Coptidis Rhizoma with multiple pharmacological activities, including anti-inflammation, promotion of apoptosis and anticancer potential effect. Mitogen-activated protein kinase (MAPK) and reactive oxygen species (ROS) may contribute to the causal relationship between tumorigenesis and pro-apoptotic function. Berberine is studied for the mechanism of its action in apoptotic pathway in human colonic carcinoma cell. Treatment of SW620 cells with 50 μM berberine resulted in activation of the caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c; whereas, the expression of BID and anti-apoptosis factor c-IAP1, Bcl-2, and Bcl-XL were decreased markedly. Berberine-induced, dose-dependent induction of apoptosis was accompanied by sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Furthermore, the induction of apoptosis was alleviated by inhibitors specific for JNK and p38. In addition, there was an increase in the cellular levels of phospho-c-Jun, FasL and t-BID in the berberine-induced apoptosis via the activation of JNK and p38 signaling modules. NAC administration, a scavenger of ROS, reversed berberine-induced apoptosis effects via inhibition of JNK, p38 and c-jun activation, and FasL and t-BID expression. These results leads us to speculate that berberine may play an apoptotic cascade in SW620 cells by activation of the JNK/p38 pathway and induction of ROS production, providing a new mechanism for berberine-induced cell death in human colon cancer cells.
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页码:719 / 728
页数:9
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