Determination of 6-Benzylaminopurine in Bean Sprouts by Capillary Electrophoresis Compared with HPLC

A simple capillary electrophoresis (CE) method based on solid-phase extraction (SPE) for the analysis of 6-Benzylaminopurine (6-BAP) was developed. Separation conditions were obtained in the sodium phosphate buffer solution (20 mM and pH 2.0), with 25 kV applied voltage and UV detection at 274 nm. Amiloride hydrochloride (AM) was used as an internal standard. Under the selected conditions, electrophoretic analysis was completed in less than 7 min, with LOD and LOQ of 19 and 65 ng mL−1 for 6-BAP. The method was successfully applied to determine 6-BAP in bean sprouts with RSD lower than 3.6 % and the recoveries from 95.0 to 103.4 % for the spiked samples. Comparative experiments were also carried out with HPLC-UV method described in National Standards of People’s Republic of China. The validation results of the two methods are comparable, but the proposed CE method was found to be simple, rapid, allowing separation and analyte quantification in shorter time, and the reagent consumption was reduced.