TaqMan based real time PCR method for quantitative detection of basmati rice adulteration with non-basmati rice

被引：0
|
作者：
S. Joseph Lopez
机构：
[1] SGS India Private Limited,Department of Biotechnology
来源：
European Food Research and Technology | 2008年 / 227卷
关键词：
Basmati; Real time PCR; Betaine aldehyde dehydrogenase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A TaqMan based real time PCR method is developed for the first time to check the adulteration in basmati rice. Betaine aldehyde dehydrogenase 2 (BAD2) gene specific primers and probes were used to detect adulterant non-basmati rice content in basmati samples. The results obtained with market samples and validation samples (basmati rice spiked with non-basmati rice in different proportions) clearly revealed that this technique is useful to detect the adulteration very accurately with 1% detection limit.
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页码：619 / 622
页数：3
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