TaqMan based real time PCR method for quantitative detection of basmati rice adulteration with non-basmati rice

被引：0

作者：

S. Joseph Lopez

机构：

[1] SGS India Private Limited,Department of Biotechnology

来源：

European Food Research and Technology | 2008年 / 227卷

关键词：

Basmati; Real time PCR; Betaine aldehyde dehydrogenase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A TaqMan based real time PCR method is developed for the first time to check the adulteration in basmati rice. Betaine aldehyde dehydrogenase 2 (BAD2) gene specific primers and probes were used to detect adulterant non-basmati rice content in basmati samples. The results obtained with market samples and validation samples (basmati rice spiked with non-basmati rice in different proportions) clearly revealed that this technique is useful to detect the adulteration very accurately with 1% detection limit.

引用

页码：619 / 622

页数：3

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