Cloning, expression and characterization of meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae

被引：0
|
作者：
Gen Lin Zhang
Cui Wei Wang
Chun Li
机构：
[1] Beijing Institute of Technology,School of Life Science
[2] Shihezi University,Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan
来源：
Biotechnology Letters | 2012年 / 34卷
关键词：
2,3-Butanediol dehydrogenase; Enzymatic properties; Expression; Substrate specificity;
D O I：
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学科分类号：
摘要：
The budC gene encoding the meso-2,3-BDH from Klebsiella pneumoniae XJ-Li was expressed in E. coli BL21 (DE3) pLys. Hypothetical amino acid sequence alignments revealed that the enzyme belongs to the short chain dehydrogenase/reductase family. After purification and refolding, the recombinant enzyme had activities of 218 U/mg for reduction of acetoin and 66 U/mg for oxidation of meso-2,3-butanediol. Highest activities were at pH 8.0 and 9.0 respectively. These are higher than other meso-2,3-butanediol dehydrogenases from K. pneumoniae. The low Km value (0.65 mM) for acetoin indicated that the enzyme can easily reduce acetoin to meso-2,3-butanediol. There were no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol and 2S,3S-2,3-butanediol, suggesting that the enzyme has a high stereospecificity for the meso-dihydric alcohol.
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页码：1519 / 1523
页数：4
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