Characterisation of recombinant unglycosylated human serum transferrin purified from Saccharomyces cerevisiae

被引：0
作者：
Peter J. Sargent
Sebastien Farnaud
Richard Cammack
Heinz M. P. Zoller
Robert W. Evans
机构：
[1] King’s College London,Metalloprotein Research Group, Randall Division of Cell and Molecular Biophysics
[2] King’s College London,Pharmaceutical Sciences Research Division
[3] University of Cambridge,Department of Medicine
[4] Addenbrooke’s Hospital,undefined
来源：
Biometals | 2006年 / 19卷
关键词：
characterisation; iron; recombinant; transferrin; yeast;
D O I：
暂无
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学科分类号：
摘要：
Structural identity between a recombinant transferrin mutant (N413Q, N611Q) secreted from Saccharomyces cerevisiae and the native protein was shown by CD analysis and immunodiffusion assays against anti-hSTf. The ability of the recombinant protein to bind iron was confirmed by urea–PAGE and EPR analysis of the iron-saturated protein revealed the characteristic holo-transferrin spectrum, indicating conservation of both iron-binding sites. The integrity of the unglycosylated recombinant protein indicates that such protein could be a valuable tool not only for structure–function characterisation but also crystallisation assays. In addition, the recombinant transferrin was found to be as effective as native transferrin as a growth factor in cell culture medium.
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页码：513 / 519
页数：6
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