Study on the Spatial Architecture of p53, MDM2, and p14ARF Containing Complexes

被引：0
作者：
Andrej Savchenko
Mariya Yurchenko
Boris Snopok
Elena Kashuba
机构：
[1] NASU,V. Lashkaryov Institute of Semiconductor Physics
[2] NASU,R. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
[3] Karolinska Institute,Department of Microbiology, Tumor, and Cell Biology (MTC)
[4] Karolinska Institute,IRIS (Center for Integrative Recognition in the Immune System)
来源：
Molecular Biotechnology | 2009年 / 41卷
关键词：
Supramolecular protein complexes; SPR; Spatial organization; Ternary complex; Co-localization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed a surface plasmon resonance (SPR)-based immunocapture approach to study multimeric protein–protein complexes. A composition and spatial architecture of protein complexes that contained GST-tagged p53, p14ARF, and MDM2 was examined by the developed approach. Obtained results verified that the p53 protein possesses two binding sites for MDM2. Ternary complexes containing p14ARF, MDM2, and p53 proteins could only be formed when MDM2 protein functions as a bridging molecule. That was confirmed by immunoprecipitation and immunostaining.
引用
收藏
相关论文
共 140 条
  • [1] Momand J(1992)The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation Cell 6 1237-1245
  • [2] Zambetti GP(1999)The p53 pathway The Journal of Pathology 187 112-126
  • [3] Olson DC(2003)The MDM2–p53 interaction Molecular Cancer Research 1 1001-1008
  • [4] George D(1995)Complex structure and regulation of the P16 (MTS1) locus Cancer Research 55 2988-2994
  • [5] Levine AJ(1995)A novel p16INK4A transcript Cancer Research 55 2995-2997
  • [6] Prives C(1998)ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways Cell 92 725-734
  • [7] Hall PA(1998)Functional and physical interactions of the ARF tumor suppressor with p53 and Mdm2 Proceedings of the National Academy of Sciences of the United States of America 95 8292-8297
  • [8] Moll UM(2003)EBV-encoded EBNA-5 associates with P14ARF in extranucleolar inclusions and prolongs the survival of P14ARF-expressing cells International Journal of Cancer 105 644-653
  • [9] Petrenko O(2003)p14ARF induces the relocation of HDM2 and p53 to extranucleolar sites that are targeted by PML bodies and proteasomes Molecular Cancer 2 18-2073
  • [10] Stone S(2006)SPR based immuno-capturing approach for interfacial sensing architecture: mapping of MRS18–2 binding site on retinoblastoma protein Analytical and Bioanalytical Chemistry 386 2063-28458
12345678910