Comparative study of pathogenic and non-pathogenic Escherichia coli outer membrane vesicles and prediction of host-interactions with TLR signaling pathways

被引：11

作者：

Behrouzi A. ^{[1
,2
]}

Vaziri F. ^{[1
,2
]}

Riazi Rad F. ^{[3
]}

Amanzadeh A. ^{[4
]}

Fateh A. ^{[1
,2
]}

Moshiri A. ^{[1
,2
]}

Khatami S. ^{[5
]}

Siadat S.D. ^{[1
,2
]}

机构：

[1] Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran

[2] Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran

[3] Department of Immunology, Pasteur Institute of Iran, Tehran

[4] National Cell Bank of Iran, Pasteur Institute of Iran, Tehran

[5] Department of Biochemistry of Iran, Pasteur Institute of Iran, Tehran

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Escherichia coli; Outer membrane vesicles; Signaling; Toll like receptor;

D O I：

10.1186/s13104-018-3648-3

中图分类号：

学科分类号：

摘要：

Objective: The intestine is the major defensive barrier in the body by having more than 60% of the immune cells in the intestinal mucosa. The aim of this study was to evaluate the Toll like receptor (TLR) signaling pathways and immune response profiles, against outer membrane vesicles (OMVs) in pathogenic and non-pathogenic strains of Escherichia coli. Results: Our results demonstrated that despite inducing inflammatory and regulatory responses to OMVs released by both strains, there is a remarkable difference in the nature and severity of these responses between the two strains. Following the production and release of OMV by the pathogenic strain, the expressions of the pro-inflammatory cytokines were significantly elevated, in comparison to the non-pathogenic strains. Eventually, our findings suggest that OMV released by the pathogen strain might be colonized, causing inflammation, eliminating the tight junctions of epithelial cells and damaging underlying cells, without the presence of IL-17 at the inflammation site. This could have happened to prevent the development of more severe inflammation, which could lead to the inhibition of colonization. The production of IL-10 is also preventing such inflammations. On the other hand, OMV released by non-pathogenic E. coli appears to influence intestinal homeostasis by causing more anti-inflammatory responses and mild inflammation. © 2018 The Author(s).

引用

共 37 条

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