GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin

被引:0
作者
Lucila H. Silva
Meiricris T. Silva
Rita M. Gutierrez
Talita C. Conte
Cláudio A. Toledo
Marcelo S. Aoki
Richard E. Liebano
Elen H. Miyabara
机构
[1] University of São Paulo,Department of Anatomy, Biomedical Sciences Institute
[2] University of São Paulo City,Neuroscience Research Nucleus
[3] University of São Paulo,School of Arts, Sciences and Humanities
来源
Lasers in Medical Science | 2012年 / 27卷
关键词
Gallium arsenide; Low-level laser therapy; Skeletal muscle; Regeneration;
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摘要
This work investigated the effect of gallium arsenide (GaAs) irradiation (power: 5 mW; intensity: 77.14 mW/cm2, spot: 0.07 cm2) on regenerating skeletal muscles damaged by crotoxin (CTX). Male C57Bl6 mice were divided into six groups (n = 5 each): control, treated only with laser at doses of 1.5 J or 3 J, CTX-injured and, CTX-injured and treated with laser at doses of 1.5 J or 3 J. The injured groups received a CTX injection into the tibialis anterior (TA) muscle. After 3 days, TA muscles were submitted to GaAs irradiation at doses of 1.5 or 3 J (once a day, during 5 days) and were killed on the eighth day. Muscle histological sections were stained with hematoxylin and eosin (H&E) in order to determine the myofiber cross-sectional area (CSA), the previously injured muscle area (PIMA) and the area density of connective tissue. The gene expression of MyoD and myogenin was detected by real-time PCR. GaAs laser at a dose of 3 J, but not 1.5 J, significantly increased the CSA of regenerating myofibers and reduced the PIMA and the area density of intramuscular connective tissue of CTX-injured muscles. MyoD gene expression increased in the injured group treated with GaAs laser at a dose of 1.5 J. The CTX-injured, 3-J GaAs laser-treated, and the CTX-injured and treated with 3-J laser groups showed an increase in myogenin gene expression when compared to the control group. Our results suggest that GaAs laser treatment at a dose of 3 J improves skeletal muscle regeneration by accelerating the recovery of myofiber mass.
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页码:993 / 1000
页数:7
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共 142 条
  • [1] Hawke TJ(2001)Myogenic satellite cells: physiology to molecular biology J Appl Physiol 91 534-551
  • [2] Garry DJ(2010)Regulatory factors and cell populations involved in skeletal muscle regeneration J Cell Physiol 224 7-16
  • [3] Ten Broek RW(1971)Effect of laser rays on wound healing Am J Surg 122 532-535
  • [4] Grefte S(1985)The biomedical effects of laser application Lasers Surg Med 5 31-39
  • [5] Von den Hoff JW(1996)Promotion of bone repair in the cortical bone of the tibia in rats by low-energy laser (He-Ne) irradiation Calcif Tissue Int 59 297-300
  • [6] Mester E(1998)Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats Lasers Surg Med 22 97-102
  • [7] Spiry T(1992)New trend in neuroscience: low-power laser effect on peripheral and central nervous system (basic science, preclinical and clinical studies) Neurol Res 14 2-11
  • [8] Szende B(1993)He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner J Neurocytol 22 185-190
  • [9] Tota JG(1994)Enhancement of angiogenesis in regenerating gastrocnemius muscle of the toad ( Anat Embryol (Berl) 190 597-602
  • [10] Mester E(1995)) by low-energy laser irradiation Anat Rec 241 123-128