MQ-HCN-based pulse sequences for the measurement of 13C1′-1H1′, 13C1′-15N, 1H1′-15N, 13C1′-13C2′, 1H1′-13C2′, 13C6/8-1H6/8, 13C6/8-15N, 1H6/8-15N, 13C6-13C5, 1H6-13C5 dipolar couplings in 13C, 15N-labeled DNA (and RNA)

被引：0
作者：
Jiangli Yan
Takeshi Corpora
Padmanava Pradhan
John H. Bushweller
机构：
[1] University of Virginia,Department of Molecular Physiology and Biological Physics
来源：
Journal of Biomolecular NMR | 2002年 / 22卷
关键词：
DNA; nuclear magnetic resonance (NMR); RNA;
D O I：
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摘要：
A suite of multiple quantum (MQ) HCN-based pulse sequences has been developed for the purpose of collecting dipolar coupling data in labeled nucleic acids. All the pulse sequences are based on the robust MQ-HCN experiment which has been utilized for assignment purposes in labeled nucleic acids for a number of years and provides much-needed resolution for the dipolar coupling measurements. We have attempted to collect multiple couplings centered on the 13C1′ and 13C6/8 positions. Six pulse sequences are described, one each for measurement of one-bond 13C1′-1H1′ and 13C6/8-1H6/8 couplings, one for measurement of one-bond 13C1′-15N and two-bond 1H1′-15N couplings, one for measurement of one-bond13C6/8-15N and two-bond 1H6/8-15N couplings, one for measurement of one-bond 13C1′-13C2′ and two-bond 1H1′-13C2′ couplings, and one for measurement of one-bond 13C6-13C5 and two-bond 1H6-13C5 couplings in the bases of C and T. These sequences are demonstrated for a labeled 18 bp DNA duplex in a 47 kDa ternary complex of DNA, CBFβ, and the CBFα Runt domain, thus clearly demonstrating the robustness of the pulse sequences even for a very large complex.
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页码：9 / 20
页数：11
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共 88 条
[11]  
Zehr M.(1995)undefined J. Magn. Reson. 106B 300-7366
[12]  
Abildgaard F.(1997)undefined J. Am. Chem. Soc. 119 7361-6400
[13]  
Peng J.(1998)undefined J. Am. Chem. Soc. 120 6394-122
[14]  
Speck N.A.(1998)undefined J. Magn. Reson. 130 119-12182
[15]  
Bushweller J.H.(1993)undefined J. Am. Chem. Soc. 115 12181-727
[16]  
Brutscher B.(1993)undefined J. Biomol. NMR 3 721-303
[17]  
Boisbouvier J.(1995)undefined J. Magn. Reson. A117 295-5978
[18]  
Pardi A.(1994)undefined J. Am. Chem. Soc. 116 5977-8082
[19]  
Marion D.(1997)undefined J. Am. Chem. Soc. 119 8076-1114
[20]  
Simorre J.(2000)undefined Science 278 1111-300
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