Cloning, functional expression and promoter analysis of xylanase III gene from Trichoderma reesei

被引：0
作者：
W. Ogasawara
Y. Shida
T. Furukawa
R. Shimada
S. Nakagawa
M. Kawamura
T. Yagyu
A. Kosuge
J. Xu
M. Nogawa
H. Okada
Y. Morikawa
机构：
[1] Shinshu University,Department of Applied Biology, Faculty of Textile Science and Technology
[2] Nagaoka University of Technology,Department of Bioengineering
来源：
Applied Microbiology and Biotechnology | 2006年 / 72卷
关键词：
Cellulase; Glycoside Hydrolase Family; Birchwood Xylan; Cellulase Gene; Xylanase Gene;
D O I：
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摘要：
In this study, the xyn3 gene from the filamentous mesophilic fungus Trichoderma reesei (Hypocrea jecorina) PC-3-7 was cloned and sequenced. Analysis of the deduced amino acid sequence of XYN III revealed considerable homology with xylanases belonging to glycoside hydrolase family 10. These results show that XYN III is distinguishable from XYN I and XYN II, two other T. reesei xylanases that belong to the glycosidase family 11. When xyn3 was expressed in Escherichia coli, significant activity was observed in the cell-free extract, and higher activity (13.2 U/ml medium) was recovered from the inclusion bodies in the cell debris. The sequence of the 5′-upstream region of the gene in the parent strain QM9414 is identical to that of PC-3-7, although the expression level of xyn3 in PC-3-7 has been reported to be at least 1,000 times greater than in QM9414. These results suggest that xyn3 expression in T. reesei QM9414 is silenced. The consensus sequences for ACEI, ACEII, CREI, and the Hap2/3/5 protein complex are all present in the upstream region of xyn3. Deletion analysis of the upstream region revealed that two regions containing consensus sequences for the known regulatory elements play important roles for xyn3 expression.
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页码：995 / 1003
页数：8
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