A simple method for site-directed mutagenesis with double-stranded plasmid DNA

被引:0
作者
Derhsing Lai
Xuli Zhu
Sidney Pestka
机构
[1] Allegheny University of the Health Sciences,Center for Neural Virology and Neural Oncology
[2] Celgene,Department of Molecular Genetics and Microbiology
[3] University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School,undefined
来源
Molecular Biotechnology | 1998年 / 9卷
关键词
Polymerase Chain Reaction Method; Klenow Fragment; Mutagenic Primer; Mutant Plasmid; Amplification Refractory Mutation System;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
页码:235 / 241
页数:6
相关论文
共 66 条
[1]  
Derbyshire K. M.(1986)A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides Gene 46 145-152
[2]  
Salvo J. J.(1989)Creation of phosphorylation sites in proteins: construction of a phosphorylatable human interferon α Proc. Natl. Acad. Sci. USA 86 558-562
[3]  
Grindley N. D. F.(1988)A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions Nucleic Acids Res. 16 7351-7367
[4]  
Li B.(1989)Construction of mutant and chimeric genes using the poly-merase chain reaction Nucleic Acids Res. 17 723-733
[5]  
Langer J. A.(1989)Use of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis Gene 76 161-166
[6]  
Schwartz B.(1989)Site-directed mutagenesis by overlap extension using the polymerase chain reaction Gene 77 51-59
[7]  
Pestka S.(1984)Improvement of oligonucleo-tide-directed site-specific mutagenesis using double-stranded plasmid DNA Bio/Technology 2 636-639
[8]  
Hignichi R.(1986)Procedures for in vitro DNA mutagenesis of human leukocyte interferon sequences Methods Enzymol. 119 403-415
[9]  
Krummel B.(1982)Oligonucleo-tide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment Nucleic Acids Res. 10 6487-6500
[10]  
Saiki R. K.(1990)A rapid method for site-specific mutagenesis and directional subcloning by using the polymerase chain reaction to generate recombinant circles BioTechniques 8 178-183