Epigenetic modification regulates both expression of tumor-associated genes and cell cycle progressing in human colon cancer cell lines: Colo-320 and SW1116

被引：0
作者：
Jing Yuan FANG
Ying Xuan CHEN
Juan LU
Rong LU
Li YANG
Hong Yin ZHU
Wei Qi GU
Lun Gen LU
机构：
[1] Shanghai Institute of Digestive Disease,
[2] Renji Hospital Shanghai Second Medical University,undefined
来源：
Cell Research | 2004年 / 14卷
关键词：
human colon cancer cell lines; tumor-associated genes; DNA methylation; histone acetylation; cell cycle;
D O I：
暂无
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学科分类号：
摘要：
The aim of this study is to assess the effects of DNA methylation and histone acetylation, alone or in combination, on the expression of several tumor-associated genes and cell cycle progression in two established human colon cancer cell lines: Colo-320 and SW1116. Treatments with 5-aza-2'-deoxycytidine (5-aza-dC) and trichostatin A, alone or in combination, were applied respectively. The methylation status of the CDKN2A promoter was determined by methylation-specific PCR, and the acetylated status of the histones associated with the p21WAF1 and CDKN2A genes was examined by chromatin immunoprecipitation. The expression of the CDKN2A, p21WAF1, p53, p73, APC, c-myc, c-Ki-ras and survivin genes was detected by real-time RT-PCR and RT-PCR. The cell cycle profile was established by flow cytometry.
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页码：217 / 226
页数：9
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