Rosmarinic acid production by Lavandula vera MM cell-suspension culture

被引：0

作者：

M. Ilieva

A. Pavlov

机构：

[1] Bulgarian Academy of Sciences,

[2] Institute of Microbiology,undefined

[3] Laboratory of Biotechnology and Industrial Microbiology,undefined

[4] 26 Maritza Blvd.,undefined

[5] B-4002 Plovdiv,undefined

[6] Bulgaria,undefined

来源：

Applied Microbiology and Biotechnology | 1997年 / 47卷

关键词：

Nitrogen; Biomass; Sucrose; Cell Culture; Phosphorus;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The time courses of growth and rosmarinic acid production by Lavandula vera MM cell suspension were investigated. The uptake of the main nutrients (sucrose, nitrogen, phosphorus, K, Ca, Mg) was followed during cultivation and the data on the physiology of the L. vera MM cell culture are presented. It was established that the cell culture synthesizes rosmarinic acid during the linear phase of growth for a relatively short period (between the 4th and 8th days of cultivation). The influence of sucrose concentration in the nutrient medium on cell growth and accumulation of rosmarinic acid by L. vera MM cell culture was investigated. The results showed that 7% sucrose in the nutrient medium ensured a steady growth of the cell suspension and increased the yield of rosmarinic acid (29.2 g/l dry biomass and 507.5 mg/l rosmarinic acid compared to 13.0 g/l dry biomass and 68.6 mg/l rosmarinic acid for the control cultivation with 3% sucrose).

引用

页码：683 / 688

页数：5