Stabilization of ATP reagents containing firefly L. mingrelica luciferase by polyols

被引：3

作者：

Moroz N.A. ^{[1
]}

Gurskii D.Ya. ^{[1
]}

Ugarova N.N. ^{[1
]}

机构：

[1] Department of Chemical Enzymology,

来源：

Moscow University Chemistry Bulletin | 2008年 / 63卷 / 2期

关键词：

Polyol; Magnesium Sulfate; Original Activity; Luciferin; Inactivation Rate;

D O I：

10.3103/S0027131408020041

中图分类号：

学科分类号：

摘要：

ATP reagents containing 2-20% of ethylene glycol, glycerol, or PEG-8000 have been prepared. Polyols inhibit the activity of the ATP reagents by 20-40%, and addition of 0.75 mM Triton X-100 prevents this inhibition. The optimal polyol concentration is 10%. The kinetics of thermoinactivation of the ATP reagents in solution at 37, 20, and 4°C has been studied. At all temperatures, the stability of ATP reagents increases two-to fivefold in the presence of polyols. A solution of ATP reagents kept at 4°C for 180 h retains 100% activity. The activity half-life is 15 days. © 2008 Allerton Press, Inc.

引用

页码：67 / 70

页数：3

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