Stabilization of ATP reagents containing firefly L. mingrelica luciferase by polyols

ATP reagents containing 2-20% of ethylene glycol, glycerol, or PEG-8000 have been prepared. Polyols inhibit the activity of the ATP reagents by 20-40%, and addition of 0.75 mM Triton X-100 prevents this inhibition. The optimal polyol concentration is 10%. The kinetics of thermoinactivation of the ATP reagents in solution at 37, 20, and 4°C has been studied. At all temperatures, the stability of ATP reagents increases two-to fivefold in the presence of polyols. A solution of ATP reagents kept at 4°C for 180 h retains 100% activity. The activity half-life is 15 days. © 2008 Allerton Press, Inc.