Purification and characterization of a novel antimicrobial peptide from sheep reproductive tract

被引：0
|
作者：
Chen Chen
Chaofeng Ku
Xinwen Bo
Xinhua Wang
机构：
[1] Shaanxi University of Technology,College of Biological Science and Engineering
[2] No.1 Senior School in Lushi County,The Breed and Biotechnology Key Laboratory of Sheep
[3] Xinjiang Production and Construction Group,undefined
来源：
Biotechnology Letters | 2015年 / 37卷
关键词：
Antimicrobial peptide; Reproductive tract; Sheep; SRTAP-40; sp;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel antimicrobial peptide, SRTAP-40 has been purified and characterized from sheep reproductive tract. The isolation procedure entailed acetic acid extraction, gel filtration chromatography, and HPLC. SRTAP-40 is composed of 40 amino acid residues with a MW of 4,820.47 Da from MALDI-TOF–MS. Its N-terminal sequence was AYVLDEPKP. SRTAP-40 cDNA was cloned by 3′-RACE. SRTAP-40 showed activity against E. coliStaphylococcus aureus,Streptococcus sp. and, Candida albicans with MIC values of 12, 12, 24, 6 μg/ml, respectively. By BLAST search, SRTAP-40 had no significant similarity to any known peptide.
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页码：327 / 332
页数：5
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