Preparation of gold nanoparticles supported on graphene oxide with flagella as the template for nonenzymatic hydrogen peroxide sensing

Gold nanoparticles supported on graphene oxide with flagella as the template were developed as an electrochemical sensor for the detection of hydrogen peroxide (H2O2) in serum. The flagella–Au nanoparticles composite and graphene oxide were dropped onto a glassy carbon electrode (GCE) to form a new H2O2 electrochemical sensor. The structure morphology of the prepared sensor was characterized by transmission electron microscopy (TEM), and the electrocatalytic performance towards H2O2 reduction was evaluated by cyclic voltammetry (CV) and amperometric methods. The response current of the sensor showed a good linear relationship with the concentration of H2O2 in the range of 10–1000 μM (R2 = 0.9916). The minimum detection limit of 1 μM was obtained (S/N = 3). Finally, the sensor was applied to the detection of H2O2 in serum, and the recoveries were satisfactory. As the sensor is sensitive, fast, and easy to make, it is expected to be used for rapid detection of H2O2.