Characterization of the combined effects of enzyme, pH and temperature treatments for removal of pathogens from sewage sludge

被引:0
作者
Nagina Parmar
Ajay Singh
Owen P. Ward
机构
[1] University of Waterloo,Department of Biology
来源
World Journal of Microbiology and Biotechnology | 2001年 / 17卷
关键词
Enzyme treatment; sewage sludge; protease; pathogens; coliforms;
D O I
暂无
中图分类号
学科分类号
摘要
The combined effects of enzyme, pH and temperature treatments for removal of pathogens from sewage sludge were investigated. An adjustment to pH 10 for 24-h at 23 °C or a 3-h hold at 50 °C resulted in a 100% kill of coliforms but a significant count of Salmonella species still survived. However, pH adjustment to 12 with a hold of 48-h at 23 °C or pH adjustment to 10 or 12 and a 3-h hold at 60 °C was required to achieve a 100% kill of Salmonella species. Although protease enzyme treatment at 40 °C with or without alkali treatment eliminated coliforms completely, Salmonella counts were reduced by two to three orders of magnitude. However, complete elimination of pathogens was observed when enzymatic treatment was provided at 50 °C. Application of alkaline protease, through its combined beneficial effects in pathogen reduction, solids reduction and improved solids settling, has potential as an effective procedure for processing of sewage sludge.
引用
收藏
页码:169 / 172
页数:3
相关论文
共 25 条
[1]  
Becker T.(1983)Downstream processing of proteins Biotechnology Advances 1 247-261
[2]  
Ogez J.R.(1959)A rapid method of total lipid extraction and purification Canadian Journal of Biochemistry and Physiology 37 911-917
[3]  
Builder S.E.(1983)Risanamento igienico dei fanghi risultanti dalla depurazione delle acque reflue Nuovi Annali di igiene e Microbiologia XXXIV 223-239
[4]  
Bligh E.G.(1991)Nature of Applied and Environmental Microbiology 57 1893-1898
[5]  
Dyer WJ.(1983) cell lysis by culture supernatants of Waste Management Residues 1 157-176
[6]  
Coppola S.(1999) species Biological Agriculture and Horticulture 16 409-430
[7]  
Manfredi C.(1993)The biology of composting: a review Water Science and Technology 28 189-192
[8]  
Dean C.R.(1967)Pathogen reduction in sewage sludge by composting and other biological treatments: a review Journal of Bacteriology 94 934-941
[9]  
Ward O.P.(1991)Improved sludge dewatering by enzymatic treatment Revue Scientifique Technique Office International Epizooties 10 813-846
[10]  
De Bertoldi M.(1981)Lysis of Applied and Environmental Microbiology 41 1117-1122