Effect of nitrogen and phosphorus fertilization on the composition of rhizobacterial communities of two Chilean Andisol pastures

被引:0
作者
Milko A. Jorquera
Oscar A. Martínez
Luis G. Marileo
Jacquelinne J. Acuña
Surinder Saggar
María L. Mora
机构
[1] Universidad de La Frontera,Scientific and Technological Bioresource Nucleus
[2] Universidad Austral de Chile,Facultad de Ciencias, Instituto de Bioquímica y Microbiología
[3] Universidad de La Frontera,Programa de Doctorado en Ciencias de Recursos Naturales
[4] Landcare Research,Ecosystems and Global Change
来源
World Journal of Microbiology and Biotechnology | 2014年 / 30卷
关键词
Bacterial community; Fertilization; Pasture; Rhizosphere; Andisol;
D O I
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中图分类号
学科分类号
摘要
The effect of nitrogen (N) and phosphorus (P) fertilization on composition of rhizobacterial communities of volcanic soils (Andisols) from southern Chile at molecular level is poorly understood. This paper investigates the composition of rhizobacterial communities of two Andisols under pasture after 1- and 6-year applications of N (urea) and P (triple superphosphate). Soil samples were collected from two previously established sites and the composition of rhizobacterial communities was determined by denaturing gradient gel electrophoresis (PCR–DGGE). The difference in the composition and diversity between rhizobacterial communities was assessed by nonmetric multidimensional scaling (MDS) analysis and the Shannon–Wiener index. In Site 1 (fertilized for 1 year), PCR–DGGE targeting 16S rRNA genes and MDS analysis showed that moderate N application (270 kg N ha−1 year−1) without P significantly changed the composition of rhizobacterial communities. However, no significant community changes were observed with P (240 kg P ha−1 year−1) and N–P application (270 kg N ha−1 year−1 plus 240 kg P ha−1 year−1). In Site 2 (fertilized for 6 years with P; 400 kg P ha−1 year−1), PCR–DGGE targeting rpoB, nifH, amoA and alkaline phosphatase genes and MDS analysis showed changes in rhizobacterial communities only at the highest rate of N application (600 kg N ha−1 year−1). Quantitative PCR targeting 16S rRNA genes also showed higher abundance of bacteria at higher N application. In samples from both sites, the Shannon–Wiener index did not show significant difference in the diversity of rhizobacterial communities. The changes observed in rhizobacterial communities coincide in N fertilized pastures with lower soil pH and higher pasture yields. This study indicates that N–P application affects the soil bacterial populations at molecular level and needs to be considered when developing fertilizer practices for Chilean pastoral Andisols.
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页码:99 / 107
页数:8
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