Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic

被引:0
作者
Bo Song
Yan-Jun Rong
Ming-Xin Zhao
Zhen-Ming Chi
机构
[1] Ocean University of China,Unesco Chinese Center of Marine Biotechnology
来源
Applied Microbiology and Biotechnology | 2013年 / 97卷
关键词
Fungal pathogen; Lipopeptide; Surfactin;
D O I
暂无
中图分类号
学科分类号
摘要
The bacterium Bacillus amyloliquefaciens anti-CA isolated from mangrove system was found to be able to actively kill Candida albicans isolated from clinic. The bacterial strain anti-CA could produce high level of bioactive substance, amylase and protease in the cheap medium containing 2.0 % soybean meal, 2.0 % wheat flour, pH 6.5 within 26 h. After purification, the main bioactive substance was confirmed to be a cyclic lipopeptide containing a heptapeptide, L-Asp→L-Leu→L-Leu→L-Val→L-Val→L-Glu→L-Leu and a 3-OH fatty acid (15 carbons). In addition to C. albicans, the purified lipopeptide can also kill many yeast strains including Metschnikowia bicuspidata, Candida tropicalis, Yarrowialipolytica and Saccharomyces cerevisiae. After treated by the purified lipopeptide, both the whole cells and protoplasts of C. albicans were destroyed.
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页码:7141 / 7150
页数:9
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