Detection of Leptosphaeria maculans from imported Canola seeds

被引：13

作者：

Chen, G. Y. ^{[1
]}

Wu, C. P. ^{[1
,2
]}

Li, B. ^{[2
]}

Su, H. ^{[2
]}

Zhen, S. Z. ^{[2
]}

An, Y. L. ^{[2
]}

机构：

[1] Nanjing Forest Univ, Jiangsu Prov Key Lab Prevent & Management Invas S, Nanjing, Peoples R China

[2] Jiangsu Entry Exit Inspect & Quarantine Bur, Plant Quarantine Lab, Nanjing, Peoples R China

来源：

JOURNAL OF PLANT DISEASES AND PROTECTION | 2010年 / 117卷 / 04期

关键词：

blackleg; canola; Leptosphaeria; quarantine; PHOMA STEM CANKER; POLYMERASE-CHAIN-REACTION; OILSEED RAPE; PCR; EPIDEMIOLOGY; BLACKLEG; PRIMERS; CHINA;

D O I：

10.1007/BF03356356

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Leptosphaeria maculans, the causal agent of blackleg on oilseeds in many regions around the world, has not been reported in China. In this study, L. maculans was detected from imported canola seeds by polymerase chain reaction (PCR) using a species-specific primer pair. The isolated L. maculans exhibited morphology identical to the known L. maculans strain on PDA culture plates and produced typical blackleg symptom when inoculated on oilseed leaves. It is the first report on the detection and identification of L. maculans from Canadian oilseed in China.

引用

页码：173 / 176

页数：4

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