Neutrophil extracellular traps and neutrophil-derived mediators as possible biomarkers in bronchial asthma

被引:0
|
作者
Gilda Varricchi
Luca Modestino
Remo Poto
Leonardo Cristinziano
Luca Gentile
Loredana Postiglione
Giuseppe Spadaro
Maria Rosaria Galdiero
机构
[1] University of Naples Federico II,Department of Translational Medical Sciences
[2] University of Naples Federico II,Center for Basic and Clinical Immunology Research (CISI)
[3] World Allergy Organization (WAO) Center of Excellence,Institute of Experimental Endocrinology and Oncology (IEOS)
[4] National Research Council,Department of Molecular Medicine and Medical Biotechnologies
[5] University of Naples Federico II,undefined
来源
Clinical and Experimental Medicine | 2022年 / 22卷
关键词
Asthma; Myeloperoxidase; Neutrophil extracellular traps; ROS;
D O I
暂无
中图分类号
学科分类号
摘要
Neutrophils (PMNs) contain and release a powerful arsenal of mediators, including several granular enzymes, reactive oxygen species (ROS) and neutrophil extracellular traps (NETs). Although airway neutrophilia is associated with severity, poor response to glucocorticoids and exacerbations, the pathophysiological role of neutrophils in asthma remains poorly understood. Twenty-four patients with asthma and 22 healthy controls (HCs) were prospectively recruited. Highly purified peripheral blood neutrophils (> 99%) were evaluated for ROS production and activation status upon stimulation with lipopolysaccharide (LPS), N-formylmethionyl-leucyl-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA). Plasma levels of myeloperoxidase (MPO), CXCL8, matrix metalloproteinase-9 (MMP-9), granulocyte–monocyte colony-stimulating factor (GM-CSF) and vascular endothelial growth factor (VEGF-A) were measured by ELISA. Plasma concentrations of citrullinated histone H3 (CitH3) and circulating free DNA (dsDNA) were evaluated as NET biomarkers. Activated PMNs from asthmatics displayed reduced ROS production and activation status compared to HCs. Plasma levels of MPO, MMP-9 and CXCL8 were increased in asthmatics compared to HCs. CitH3 and dsDNA plasma levels were increased in asthmatics compared to controls and the CitH3 concentrations were inversely correlated to the % decrease in FEV1/FVC in asthmatics. These findings indicate that neutrophils and their mediators could have an active role in asthma pathophysiology.
引用
收藏
页码:285 / 300
页数:15
相关论文
共 50 条
  • [11] Neutrophil extracellular traps and neutrophil-related mediators in human thyroid cancer
    Modestino, Luca
    Cristinziano, Leonardo
    Poto, Remo
    Ventrici, Annagioia
    Trocchia, Marialuisa
    Ferrari, Silvia Martina
    Fallahi, Poupak
    Paparo, Sabrina Rosaria
    Marone, Gianni
    Antonelli, Alessandro
    Varricchi, Gilda
    Galdiero, Maria Rosaria
    FRONTIERS IN IMMUNOLOGY, 2023, 14
  • [12] Characteristics and Role of Neutrophil Extracellular Traps in Asthma
    Fei Chen
    Min Yu
    Yonghong Zhong
    Lina Wang
    Huaqiong Huang
    Inflammation, 2022, 45 : 6 - 13
  • [13] Characteristics and Role of Neutrophil Extracellular Traps in Asthma
    Chen, Fei
    Yu, Min
    Zhong, Yonghong
    Wang, Lina
    Huang, Huaqiong
    INFLAMMATION, 2022, 45 (01) : 6 - 13
  • [14] Neutrophil Extracellular Traps in Asthma: Friends or Foes?
    Poto, Remo
    Shamji, Mohamed
    Marone, Gianni
    Durham, Stephen R.
    Scadding, Guy W.
    Varricchi, Gilda
    CELLS, 2022, 11 (21)
  • [15] Neutrophil extracellular traps in bronchial aspirates: a quantitative analysis
    Hamaguchi, Shigeto
    Hirose, Tomoya
    Matsumoto, Naoya
    Akeda, Yukihiro
    Irisawa, Taro
    Seki, Masafumi
    Hosotsubo, Hideo
    Yamamoto, Kouji
    Tasaki, Osamu
    Oishi, Kazunori
    Shimazu, Takeshi
    Tomono, Kazunori
    EUROPEAN RESPIRATORY JOURNAL, 2014, 43 (06) : 1709 - 1718
  • [16] Clinical significance of neutrophil extracellular traps biomarkers in thrombosis
    Xiangbo Xu
    Yuting Wu
    Shixue Xu
    Yue Yin
    Walter Ageno
    Valerio De Stefano
    Qingchun Zhao
    Xingshun Qi
    Thrombosis Journal, 20
  • [17] Clinical significance of neutrophil extracellular traps biomarkers in thrombosis
    Xu, Xiangbo
    Wu, Yuting
    Xu, Shixue
    Yin, Yue
    Ageno, Walter
    De Stefano, Valerio
    Zhao, Qingchun
    Qi, Xingshun
    THROMBOSIS JOURNAL, 2022, 20 (01)
  • [18] NEUTROPHIL EXTRACELLULAR TRAPS AS MEDIATORS OF ENDOTHELIAL DAMAGE IN HYPERTENSION AND DIABETES
    Landry, Chloe
    Xiao, Fengxia
    Thibodeau, Jean Francois
    Holterman, Chet E.
    Xhu, Lihua
    Gutsol, Alex
    Kennedy, Christopher R. J.
    Burger, Dylan
    JOURNAL OF HYPERTENSION, 2023, 41 : E25 - E26
  • [19] NEUTROPHIL EXTRACELLULAR TRAPS
    Zychlinsky, A.
    ANNALS OF THE RHEUMATIC DISEASES, 2013, 71 : 34 - 34
  • [20] Neutrophil extracellular traps
    Zychlinsky, A.
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2009, 39 : 27 - 27