A novel second isoenzyme of the human UDP-N-acetylglucosamine:α1,3-D-mannoside β1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignmen

被引:0
作者
Aruto Yoshida
Mari T. Minowa
Shinji Takamatsu
Tomoka Hara
Hiroshi Ikenaga
Makoto Takeuchi
机构
[1] Kirin Brewery Co.,Central Laboratories for Key Technology
[2] Ltd.,undefined
来源
Glycoconjugate Journal | 1998年 / 15卷
关键词
glycosyltransferase; N-acetylglucosaminyltransferase; GnT-IV; isoenzyme; mammalian; gene family; Asn-linked oligosaccharide; chromosome 5q35;
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摘要
We isolated a novel cDNA encoding a second isoenzyme of UDP-N-acetylglucosamine:α1,3-D-mannoside β1,4-N-acetylglucosaminyltransferase (GnT-IV; EC 2.4.1.145). The nucleotide and deduced amino acid sequences of the cDNA were homologous to those of the previously cloned human GnT-IV cDNA (63% and 62% identity, respectively). The new cDNA is also confirmed to express GnT-IV activity, suggesting that two isoenzymes of human GnT-IV exist. Although genomic Southern analysis suggested that both genes exist in many mammalian species and the chicken, northern analysis revealed that both genes are expressed in different ways in human tissues. This is the first report concerning the gene family of an N-acetylglucosaminyltransferase in mammals.
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页码:1115 / 1123
页数:8
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