Biological activity of secondary metabolites produced by a strain of Pseudomonas fluorescens

被引：9

作者：

Deka Boruah H.P. ^{[1
]}

Dileep Kumar B.S. ^{[1
]}

机构：

[1] Regional Research Laboratory, Cncl. of Sci. and Indust. Research, Jorhat 785 006, Assam

来源：

Folia Microbiologica | 2002年 / 47卷 / 4期

关键词：

Antifungal Activity; Tomato Spotted Wilt Virus; Cyanic Acid; Mycobacterium Smegmatis; Fusarium Moniliforme;

D O I：

10.1007/BF02818690

中图分类号：

学科分类号：

摘要：

Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO was identified by comparing the UV-spectra and moss-green color development after 'diazotized sulfanilic acid' (DSA) spray in TLC. PHE and PHL were identified by comparing standard compounds on TLC and orange-color development immediately after DSA spray. In vitro antibiosis study of the metabolites revealed their antibacterial and antifungal activity against bacterial test organisms Corynebacterium sp., Mycobacterium phlei and M. smegmatis and test fungi Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani and Rhizoctonia solani. A statistically significantly higher plant growth was recorded in siderophore-amended plantlets under gnotobiotic conditions whereas PHE and PHL did not show any plant-growth-promoting activity. These results support the importance of the secondary metabolites produced by the strain P. fluorescens in enhancing plant growth and in controling fungal and bacterial pathogens.

引用

页码：359 / 363

页数：4

共 36 条

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