Pathological and histochemical studies of the effects of cystic echinococcosis in sheep

被引:0
作者
Beigh A.B. [1 ]
Darzi M.M. [1 ]
Bashir S. [2 ]
kashani B. [1 ]
Shah A. [1 ]
Shah S.A. [1 ]
机构
[1] Division of veterinary pathology, Faculty of Veterinary Sciences & Animal Husbandry, Shuhama, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, 190006, J&K
[2] Department of Biochemistry, Hamdard University, New Delhi
关键词
Histochemistry; Histopathology; Hydatid cyst; Sheep;
D O I
10.1007/s00580-017-2606-0
中图分类号
学科分类号
摘要
A total of 2100 sheep, slaughtered or spontaneously dead, from various areas of Kashmir valley were screened for the presence of hydatidosis. Out of 2100 cases, 85 were positive for hydatidosis. Histopathologicallly, the cyst wall consisted of the inner germinal, middle lamellated, and outer fibrous layer. Inflammatory reaction around the cyst was variable and was characterized by an inner zone of loosely arranged fibroblasts infiltrated with mononuclear cells and densely packed fibroblasts mixed with mononuclear cells; and an outer layer of concentrically packed fibrous connective tissue. Mast cell reaction was observed mainly in the bronchiolar, peribronchiolar, and inflammatory zones in the lungs; while in the liver, mast cells were noted in portal triads and bile ducts and were least evident in the vicinity of the cysts. Qualitative increase was observed for acid mucopolysaccharides in fibroblasts, inflammatory cells, and bronchial epithelium, especially hyperplastic epithelial cells. Masson’s trichrome revealed intense formation of collagen fibers in the pericystic connective tissue. The calcareous corpuscles of the protoscolices were distinct when stained with combined Alcian blue PAS and toluidine stains but were not visible when stained with H & E and Masson’s trichrome stains. © 2017, Springer-Verlag London Ltd., part of Springer Nature.
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页码:407 / 412
页数:5
相关论文
共 37 条
[1]  
Ahmad M.E., Rahim M.A., Fatima M.A., Hydatid disease, a morbid drop needs awareness, Sudan Med J, 47, (2011)
[2]  
Anwar Z., The effect of echinococcosis on rabbit and sheep along with its control by indigenous plants of Pakistan. Ph.D, Thesis, (1997)
[3]  
Anwar Z., Tanveer A., Bashir S., Echinococcus granulosus: histopathology of naturally infected sheep liver. Punjab Univ, J Zool, 14, pp. 105-111, (1999)
[4]  
Bancroft J.D., Gamble M., Theory and practice of histological techniques, pp. 181-182, (2002)
[5]  
Barnes T.S., Hinds L.A., Jenkins D.J., Bielefeldt-Ohmann H., Lightowlers M.W., Coleman G.T., Comparative pathology of pulmonary hydatid cysts in macropods and sheep, J Comp Pathol, 144, 2-3, pp. 113-122, (2011)
[6]  
Darzi M.M., Mir M.S., Khan M., Concurrent anthracosis and parasitic pneumonia in a sheep, SKUAST-J Res, 5, pp. 213-216, (2003)
[7]  
Da-Silva A., Human echinococcosis: a neglected disease, Gastroenterol Res Pract, 2010, pp. 1-9, (2010)
[8]  
Endalew D., Nurradis I., Prevalence and economic importance of echinococcosis in cattle slaughtered at North Gonder Elfora Abattoir. Eur, J Appl Sci, 5, pp. 29-35, (2013)
[9]  
Gupta V., Kaira V., Sharma J., Sen R., Sangwaiya A., Primary hydatid cyst of spleen: a rare entity, J Trop Dis, 2, (2014)
[10]  
Humason G.L., Staining cellular elements. In animal tissue techniques, fourth Edn. San Francisco, W.H. Freeman pp. 319-320, Indian J Anim Res, 37, 1, pp. 57-58, (1979)