Reversed-Phase HPLC as a method for the identification of gadoid fish species

被引：0

作者：

C. Piñeiro

Carmen G. Sotelo

Isabel Medina

José M. Gallardo

Ricardo I. Pérez-Martín

机构：

[1] Instituto de Investigaciones Marinas,

[2] Eduardo Cabello 6,undefined

[3] E-36208 Vigo,undefined

[4] Spain ,undefined

来源：

Zeitschrift für Lebensmitteluntersuchung und -Forschung A | 1997年 / 204卷

关键词：

Key words Fish species; Gadoids; Identification; HPLC; Sarcoplasmic proteins;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A simple and rapid method to identify raw or frozen gadoid fish species is described. The methodology is based on the HPLC separation of water-soluble proteins and monitoring at 230 nm. Chromatographic profiles were reproducible between individuals belonging to the same species; lower variations in the relative retention time than in peak area percentage were observed. Results obtained from an ANOVA test allowed the distinction of characteristic peaks. Profiles were independent of fish quality: fish refrigerated for 10 days showed the same HPLC protein profile as those that were fresh. The ability of the identification procedure was checked by analysis of a set of frozen commercial samples.

引用

页码：411 / 416

页数：5