Impact of Multiple Vitrification-Warming Procedures and Insemination Methods on Pregnancy and Neonatal Outcomes in Preimplantation Genetic Testing for Aneuploidy

被引：0
作者：
Qian Zhang
Wenhao Yu
Chenxi Jin
Tianxiang Ni
Tingting Zhou
Qing Zhao
Weilin Wang
Yunjia Li
Junhao Yan
机构：
[1] Shandong University,Center for Reproductive Medicine
[2] Shandong University,Key Laboratory of Reproductive Endocrinology of Ministry of Education
[3] Shandong Key Laboratory of Reproductive Medicine,National Research Center for Assisted Reproductive Technology and Reproductive Genetics
[4] Shandong Provincial Clinical Research Center for Reproductive Health,undefined
[5] Shandong Technology Innovation Center for Reproductive Health,undefined
[6] Shandong University,undefined
[7] Linyi People’s Hospital,undefined
来源：
Reproductive Sciences | 2023年 / 30卷
关键词：
PGT-A; Vitrification; IVF; ICSI; Pregnancy and neonatal outcomes;
D O I：
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中图分类号：
学科分类号：
摘要：
This study was to determine whether multiple vitrification-warming procedures and insemination method are associated with pregnancy and neonatal outcomes in preimplantation genetic testing for aneuploidy (PGT-A). This was a retrospective, single-center, observational study of 112 patients who underwent standard PGT-A practice and 154 patients who desired PGT-A for their vitrified unbiopsied blastocysts. A total of 97 euploid blastocysts biopsied and vitrified-warmed once and 117 euploid blastocysts biopsied once but vitrified-warmed twice (83 in vitro fertilization [IVF]–derived and 34 intracytoplasmic sperm injection [ICSI]–derived euploid blastocysts) were transferred. The primary outcome was the blastocyst survival rate for transfer, live birth rate, and neonatal outcomes. The results showed that an additional vitrification-warming procedure on blastocysts resulted in a lower but not statistically different survival rate for transfer. Compared with euploid blastocysts vitrified-warmed once, those vitrified-warmed twice provided statistically similar live birth rate. Neonatal outcomes, including the sex ratio, gestational age, birthweight, preterm birth rate, and low birthweight rate, did not differ between single and double vitrification. No significant differences were observed in rates of blastocyst survival, blastocyst euploid and live birth, and neonatal outcomes resulting from either conventional IVF or ICSI. The neonatal follow-up of babies live-born so far did not report any congenital malformations. In conclusion, an additional vitrification-warming on blastocysts had no detectable adverse impact on clinical outcomes after frozen-thawed single euploid blastocyst transfer in PGT-A cases; and ICSI did not confer any benefit in improving clinical outcomes compared with conventional IVF in cases requiring PGT-A on already vitrified nonbiopsied blastocysts.
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页码：2302 / 2312
页数：10
相关论文
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