Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network

被引：0
作者：
Charles Yeaman
M. Inmaculada Ayala
Jessica R. Wright
Frederic Bard
Carine Bossard
Agnes Ang
Yusuke Maeda
Thomas Seufferlein
Ira Mellman
W. James Nelson
Vivek Malhotra
机构：
[1] University of Iowa,Department of Anatomy and Cell Biology
[2] Cell and Developmental Biology,Department of Molecular and Cellular Physiology
[3] University of California San Diego,Department of Cell Biology
[4] Stanford University School of Medicine,Department of Immunoregulation
[5] Yale University,Department of Internal Medicine I
[6] Research Institute for Microbial Diseases,undefined
[7] Osaka University,undefined
[8] University of Ulm,undefined
来源：
Nature Cell Biology | 2004年 / 6卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Protein kinase D (PKD) binds to diacylglycerol (DAG) in the trans-Golgi network (TGN) and is activated by trimeric G-protein subunits βγ. This complex then regulates the formation of transport carriers in the TGN that traffic to the plasma membrane in non-polarized cells. Here we report specificity of different PKD isoforms in regulating protein trafficking from the TGN. Kinase-inactive forms of PKD1, PKD2 and PKD3 localize to the TGN in polarized and non-polarized cells. PKD activity is required only for the transport of proteins containing basolateral sorting information, and seems to be cargo specific.
引用
收藏
页码：106 / 112
页数：6
相关论文
共 83 条
  • [1] Maeda Y(2001)Recruitment of protein kinase D to the trans-Golgi network via the first cysteine-rich domain EMBO J. 20 5982-5990
  • [2] Beznoussenko GV(2002)Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane Science 295 325-328
  • [3] Van Lint J(2001)Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network Cell 104 409-420
  • [4] Mironov AA(2001)Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases J. Biol. Chem. 276 3310-3318
  • [5] Malhotra V(1999)PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu Biochim. Biophys. Acta 1450 99-106
  • [6] Baron CL(1989)Using temperature-sensitive mutants of VSV to study membrane protein biogenesis Methods Cell. Biol. 32 85-110
  • [7] Malhotra V(2000)Membrane traffic in polarized epithelial cells Curr. Opin. Cell Biol. 12 483-490
  • [8] Liljedahl M(1997)The O-glycosylated stalk domain is required for apical sorting of neurotrophin receptors in polarized MDCK cells J. Cell Biol. 139 929-940
  • [9] Sturany S(2001)Distribution and function of AP-1 clathrin adaptor complexes in polarized epithelial cells J. Cell Biol. 152 595-606
  • [10] Hayashi A(2002)AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells Nature Cell Biol. 4 154-159
123456789