Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

被引：0
作者：
Anna Shaliutina-Kolešová
Saeed Ashtiani
Mo Xian
Rui Nian
机构：
[1] University of South Bohemia in Ceske Budejovice,Department of Physical Chemistry
[2] Faculty of Fisheries and Protection of Waters,undefined
[3] South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,undefined
[4] Research Institute of Fish Culture and Hydrobiology,undefined
[5] Qingdao Institute of Bioenergy and Bioprocess Technology,undefined
[6] Chinese Academy of Sciences,undefined
[7] University of Chemistry and Technology,undefined
来源：
Fish Physiology and Biochemistry | 2020年 / 46卷
关键词：
Cyprinidae; Seminal plasma; Fractions; Quality; Sperm storage;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep® centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 ± 2.41% and 168.05 ± 4.46 μm/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 ± 1.27) and olive tail moment (0.68 ± 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.
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页码：1461 / 1468
页数：7
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