Physiological and molecular response of annual Medicago species to juglone

被引：0
作者：
Zohreh Torabi
Fariba Rafiei
Leila Shabani
Abdolrazagh Danesh Shahraki
机构：
[1] Shahrekord University,Department of Plant Breeding and Biotechnology, College of Agriculture
[2] Shahrekord University,Department of Biology, Faculty of Science
[3] Shahrekord University,Department of Agronomy, College of Agriculture
来源：
Acta Physiologiae Plantarum | 2015年 / 37卷
关键词：
Allelopathy; Antioxidant enzyme; Gene expression; Oxidative stress; Transcription factor;
D O I：
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学科分类号：
摘要：
The current study was conducted to evaluate the physiological and molecular response of self-regenerating annual Medicago species (M. polymorpha and M. lupulina) to juglone exposure. A randomized complete block design was performed in which two treatment groups consisted of a control and juglone (10−4 M) allotted to main plots and genotypes assigned to subplots. A significant increase in the concentration of GSH and the GSH/GSSG ratio was observed in both annual Medicago species in response to juglone exposure. However, such response was greater in M. lupulina than M. polymorpha. The activity of all antioxidant enzymes (CAT, APX, GST, and GPOX) was significantly increased by juglone. In response to juglone, the expression of WRKY was significantly decreased. The transcription of CBF4, Zpt2-2, CAT, and GST genes was highly induced by juglone in annual Medicago species. Higher expression of CBF4, Zpt2-2, CAT and GST genes in M. lupulina which showed more juglone-tolerance can be associated with higher tolerance against allelochemical stress. It can be concluded that M. polymorpha is juglone-sensitive because it started to show chlorosis of leaves after a week subjecting to juglone. Though M. lupulina seems to be more juglone-tolerant at least in short term, long-term exposure of juglone should also be examined to find out its suitability to grow in walnut orchards. Considering the presence of cross-talk between different stresses, we can propose that M. lupulina is more tolerant to other abiotic stresses compared to M. polymorpha.
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[1]  
Agarwal PK(2010)Transcription factors in plants and ABA dependent and independent abiotic stress signaling Biol Plantarum 54 201-212
[2]  
Jha B(2003)Allelopathy and exotic plant invasion: from molecules and genes to species interactions Science 301 1377-1380
[3]  
Bais HP(2004)Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Plant J 37 251-268
[4]  
Vepachedu R(2013)On the coexistence mechanisms of a perennial grass with an allelopathic shrub in a semiarid rangeland Int J Agri Crop Sci 5 2001-2008
[5]  
Gilroy S(1981)Glutathione-S-transferase of human red blood cells; assay, values in normal subjects and in two pathological circumstances: hyper bilirubinemia and impaired renal function Clin Chim Acta 117 209-217
[6]  
Callaway RM(2011)Identification of transcriptome profiles and signaling pathways for the allelochemical juglone in rice roots Plant Mol Biol 77 591-607
[7]  
Vivanco JM(2002)Allelochemical stress produced by the aqueous leachate of Callicarpa acuminata : effects on roots of bean, maize, and tomato Physiol Plantarum 116 20-27
[8]  
Becher M(2007)Differential expression of the TFIIIA regulatory pathway in response to salt stress between Plant Physiol 145 1521-1532
[9]  
Talke IN(2007) genotypes J Exp Bot 58 3765-3773
[10]  
Krall L(1998)Physiological basis of different allelopathic reactions of cucumber and figleaf gourd plants to cinnamic acid Curr Opin Plant Biol 1 258-266
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