Experimental evaluation of an antimicrobial protein from Bacillus amyloliquefaciens MBL27 for wound healing potential in rats

被引：0

作者：

K V. ^{[1
]}

G S.R. ^{[2
]}

机构：

[1] Department of Biochemistry, College of Science and Humanities, SRM Institute of Science and Technology, Tamil Nadu, Kattankulathur

[2] Microbiology Laboratory, Central Leather Research Institute, Adyar, Chennai

来源：

Comparative Clinical Pathology | 2022年 / 31卷 / 4期

关键词：

Animal models; Antimicrobial protein; Bacillus amyloliquefaciens; Chitosan; Collagen; Wound healing;

D O I：

10.1007/s00580-022-03361-1

中图分类号：

学科分类号：

摘要：

This study was aimed at assessing the abilityof the antimicrobial protein (AMP) produced by Bacillus amyloliquefaciens MBL27 as a potent wound healant. Ratmodels were used to study the efficacy of AMP and AMP-incorporated chitosansheet along with control groups. AMP and AMP-incorporated chitosan sheetsignificantly improved wound contraction when compared to controls. Rate ofwound contraction (97.23%), decreased period of epithelialization (14 days),and the levels of biochemical markers such as hydroxyproline (collagen), totalprotein, uronic acid, and hexosamine in the granulation tissue on differentdays of wound healing revealed the wound healing efficacy of the AMP. Thehistological examinations also correlated well with the biochemical findings,confirming the wound healing efficacy of the AMP. The results indicate thebeneficial effects of AMP from B. amyloliquefaciensMBL27 and it is prospective to be developed into novel therapeutic agent fordermal wound healing. © 2022, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.

引用

页码：627 / 647

页数：20

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