Determination of phylogenetic background, fimbrial genes, and antibiotic susceptibility of Escherichia coli isolates from urinary tract infections in Bam region, Iran

被引：3

作者：

Alizade H. ^{[1
,2
,6
]}

Ghanbarpour R. ^{[3
,4
]}

Aflatoonian M.R. ^{[1
,5
,6
]}

Abdollahi H. ^{[1
,6
,7
]}

机构：

[1] Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman

[2] Department of microbiology, Kerman University of Medical Sciences, International Campus, Kerman

[3] Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman

[4] Zeonosis Research Committee, Kerman University of Medical Sciences, Kerman

[5] Leishmaniosis Research Center, Kerman University of Medical Sciences, Kerman

[6] Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman

[7] Department of Microbiology, Kerman University of Medical Sciences, P.O. Box 7616914115, Kerman

来源：

Comparative Clinical Pathology | 2014年 / 23卷 / 5期

关键词：

Escherichia coli; Fimbriae; Phylogenetic; Urinary tract infections;

D O I：

10.1007/s00580-013-1771-z

中图分类号：

学科分类号：

摘要：

Phylogenetic analysis have shown that Escherichia coli (E. coli) strains segregate in four main phylogenetic groups A, B1, B2, and D. E. coli fimbriae increase invasive capability of bacteria to renal tissues. The purpose of this study was to determine the distribution of phylogenetic groups/subgroups among fimbrial genes and antibiotic resistance patterns of E. coli isolates from urinary tract infections (UTI), in Bam (southeast of Iran). A total of 122 E. coli isolates from patients with UTI, which were confirmed by biochemical tests, were collected. Antibiotic susceptibility of isolates was examined against six antibiotic agents by disk diffusion method. DNA was extracted and examined for detection of phylogenetic group/subgroups and also for determination of afaI BC, sfa/focDE, and papEF genes using PCR technique. E. coli isolates were distributed in phylogroups A (45.08 %), D (43.45 %), B2 (7.83 %), and B1 (4.09 %). The examined isolates belonged to six phylogenetic subgroups A0 (28.69 %), D2 (24.59 %), D1 (18.85 %), A1 (16.39 %), B2–3 (7.39 %), and B1 (4.09 %). Fimbrial genes were found in 27.85 % of isolates. Phylogroups A and D were more prevalent in antibiotic resistance patterns than other phylogenetic groups. The findings of the current study showed that A and D phylogenetic groups were dominant among our isolates. These results differ with that of other researches in other parts of the world. Further studies are required to clarify the phylogenetic background in Bam area. Antibiotic resistant seems to be a common feature of most E. coli isolates in this area. © 2013, Springer-Verlag London.

引用

页码：1253 / 1257

页数：4

共 26 条

[1]

Abdallah K.S., Cao Y., Wei D.J., Epidemiologic investigation of extraintestinal pathogenic E. coli (ExPEC) based on PCR phylogenetic group and fimH single nucleotide polymorphisms (SNPs) in China, Int J Mol Epidemiol Genet, 2, pp. 339-353, (2011)

[2]

Arancibia E.M., Pitart C., Ruiz J., Marco F., Gascon J., Vila J., Evolution of antimicrobial resistance in enteroaggregative Escherichia coli and enterotoxigenic Escherichia coli causing traveler’s diarrhea, J Antimicrob Chemoth, 64, pp. 343-347, (2009)

[3]

Arisoy M., Rad A.Y., Akin A., Akar N., Relationship between susceptibility to antimicrobials and virulence factors in pediatric Escherichia coli isolates, Int J Antimicrob Ag, 31, pp. 4-8, (2008)

[4]

Banu A., Kabbin J.S., Anand M., Extraintestinal infections due to Escherichia coli: an emerging issue, J Clin Diagn Res, 5, pp. 486-490, (2011)

[5]

Bouguenec C.L., Laliouil L., Merle L.D., Et al., Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates: PCR assays for detection of Afa adhesins that do or do not recognize Dr blood group antigens, J Clin Microbiol, 39, pp. 1738-1745, (2001)

[6]

Carlos C., Pires M.M., Stoppe N.C., Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination, BMC Microbiol, (2010)

[7]

Cheng C.H., Tsai M.H., Huang Y.C., Et al., Antibiotic resistance patterns of community acquired urinary tract infections in children with vesicoureteral reflux receiving prophylactic antibiotic therapy, Pediatrics, 122, pp. 1212-1217, (2008)

[8]

Choi U.Y., Han S.B., Lee S.Y., Kang J.H., Kim S.M., Ma S.H., Regional differences in phylogenetic group of Escherichia coli strains isolated from children with urinary tract infection in Korea, Korean J Pediatr, 55, pp. 420-423, (2012)

[9]

Clermont O., Bonacorsi S., Bingen E., Rapid and simple determination of the Escherichia coli phylogenetic group, Appl Environ Microb, 66, pp. 4555-4558, (2000)

[10]

Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement, CLSI document M100–S22, (2012)

← 1 2 3 →