Murine mCLCA5 is expressed in granular layer keratinocytes of stratified epithelia

被引：0
作者：
Josephine Braun
Melanie K. Bothe
Lars Mundhenk
Carol L. Beck
Achim D. Gruber
机构：
[1] Freie Universität Berlin,Department of Veterinary Pathology, Faculty of Veterinary Medicine
[2] Thomas Jefferson University,Department of Pharmacology and Experimental Therapeutics
来源：
Histochemistry and Cell Biology | 2010年 / 133卷
关键词：
Calcium-activated chloride channel; Squamous epithelium; Mouse; Skin; Stomach;
D O I：
暂无
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学科分类号：
摘要：
CLCA proteins represent a large family of proteins widely expressed in mammalian tissues with a unique expression pattern for each family member analyzed so far. However, their functions in normal and diseased tissues are poorly understood. Here, we present the cellular expression pattern of mCLCA5 in murine tissues using immunohistochemistry, confocal laser scanning microscopy and immune electron microscopy with specific antibodies and RT-qPCR following laser-capture microdissection. The mCLCA5 protein was localized to granular layer keratinocytes of virtually all stratified squamous epithelia of the body. Biochemical protein characterizations revealed that the amino-terminal cleavage product is fully secreted by the cell, while the carboxy-terminal cleavage product remains associated with the cell. The results imply that mCLCA5 may play a role in maturation and keratinization of squamous epithelial cells.
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页码：285 / 299
页数：14
相关论文
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