Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

被引：0
|
作者：
Madhu S. Budamagunta
John F. Hess
Paul G. Fitzgerald
John C. Voss
机构：
[1] University of California Davis,Department of Biochemistry & Molecular Medicine
[2] University of California Davis,Department of Cell Biology and Human Anatomy, School of Medicine
来源：
Cell Biochemistry and Biophysics | 2007年 / 48卷
关键词：
Intermediate filaments; Vimentin; EPR (ESR) spectroscopy; Site-directed spin labeling;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
引用
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页码：45 / 53
页数：8
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