Identification and characterization of conserved and novel microRNAs in Xanthoceras sorbifolium via deep sequencing

被引：0
作者：
Quanxin Bi
Bin Guo
Dongxu Zhang
Wenbin Guan
机构：
[1] Beijing Forestry University,School of Nature Conservation
[2] Longtan Park,Crops Institute of Alpine Region
[3] Datong University,undefined
来源：
Genes & Genomics | 2015年 / 37卷
关键词：
MicroRNA; qRT-PCR; Deep sequencing;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Xanthoceras sorbifolium is an economically important woody plant in China. Plant microRNAs (miRNAs) play a vital role in plant development. We used Solexa technology to sequence a small RNA library from the buds, young and mature leaves, flowers, and developing seeds (10, 30, and 60 days after pollination) of X. sorbifolium to identify conserved and novel miRNAs. Sequence analysis identified 49 conserved miRNAs belonging to 19 miRNA families, as well as four novel miRNAs. An analysis of 10 miRNA sequences by qRT-PCR showed that all sequences were expressed and that they were dynamically regulated in different tissues. Finally, seven targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the roles of miRNAs in X. sorbifolium.
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页码：281 / 286
页数：5
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