Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry

被引：7
|
作者：
Pometun A.A. [1 ,2 ]
Kleymenov S.Y. [1 ,4 ]
Zarubina S.A. [2 ,3 ]
Kargov I.S. [1 ,2 ,3 ]
Parshin P.D. [2 ,3 ]
Sadykhov E.G. [1 ]
Savin S.S. [2 ,3 ]
Tishkov V.I. [1 ,2 ,3 ]
机构：
[1] Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”, Russian Academy of Sciences, Moscow
[2] Innovations and High Technologies MSU Ltd., Moscow
[3] Department of Chemistry, Moscow State University, Moscow
[4] Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow
来源：
Moscow University Chemistry Bulletin | 2018年 / 73卷 / 2期
基金：
俄罗斯基础研究基金会; 俄罗斯科学基金会;
关键词：
differential scanning calorimetry; formate dehydrogenase; thermal stability;
D O I：
10.3103/S002713141802013X
中图分类号：
学科分类号：
摘要：
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH), and moss Physcomitrella patens (PpaFDH) have been cloned and expressed in E. coli cells. By means of differential scanning calorimetry, a comparative study of thermal stability of new recombinant formate dehydrogenases and a number of FDHs from other sources has been performed. It was shown that two new enzymes, SauFDH and OpaFDH, are comparable to the FDH from Pseudomonas sp. 101 bacteria in their stability. SceFDH is the least stable FDH among the described formate dehydrogenases. © 2018, Allerton Press, Inc.
引用
收藏
页码：80 / 84
页数：4
相关论文
共 50 条
  • [1] Differential scanning calorimetry study of haemin thermal stability
    Z. Drzazga
    A. Michnik
    M. Zimnicka
    Journal of Thermal Analysis and Calorimetry, 2003, 72 : 555 - 563
  • [2] Thermal Stability Evaluation of Nitroalkanes with Differential Scanning Calorimetry
    Maule, Ivano
    Razzetti, Gabriele
    Restelli, Alessandro
    Palmieri, Alessandro
    Colombo, Cinzia
    Ballini, Roberto
    ORGANIC PROCESS RESEARCH & DEVELOPMENT, 2021, 25 (04) : 781 - 788
  • [3] Differential scanning calorimetry study of haemin thermal stability
    Drzazga, Z
    Michnik, A
    Zimnicka, M
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, 2003, 72 (02) : 555 - 563
  • [4] THERMAL-STABILITY OF MOTOR OILS - DIFFERENTIAL SCANNING CALORIMETRY
    LOVASZ, C
    ERDOL & KOHLE ERDGAS PETROCHEMIE, 1977, 30 (05): : 219 - 223
  • [5] THERMAL AND STABILITY STUDY OF TETRACENE USING DIFFERENTIAL SCANNING CALORIMETRY
    NORWITZ, G
    EVERETT, ME
    GURBARG, MT
    THERMOCHIMICA ACTA, 1975, 12 (03) : 227 - 232
  • [6] ASSESSING COLLAGEN THERMAL-STABILITY BY DIFFERENTIAL SCANNING CALORIMETRY
    JUDGE, MD
    MILLS, EW
    FLEISCHWIRTSCHAFT, 1986, 66 (09): : 1417 - 1419
  • [7] Study of the Thermal Stability of Petroleum Asphaltenes by Differential Scanning Calorimetry
    Korneev, Dmitry S.
    Pevneva, Galina S.
    Golovko, Anatoly K.
    PROCEEDINGS OF THE ADVANCED MATERIALS WITH HIERARCHICAL STRUCTURE FOR NEW TECHNOLOGIES AND RELIABLE STRUCTURES, 2018, 2051
  • [8] Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry
    Malicka, Weronika
    Dai, Yueyue
    Herrmann, Andreas
    Haag, Rainer
    Ballauff, Matthias
    Pigaleva, Marina
    Risse, Thomas
    Lauster, Daniel
    Asakereh, Iman
    Khajehpour, Mazdak
    CHEMISTRYOPEN, 2025,
  • [9] Practical Use of Differential Scanning Calorimetry for Thermal Stability Hazard Evaluation
    Sheng, Min
    Valco, Daniel
    Tucker, Craig
    Cayo, Elizabeth
    Lopez, Tyler
    ORGANIC PROCESS RESEARCH & DEVELOPMENT, 2019, 23 (10) : 2200 - 2209
  • [10] The effect of toxins on the thermal stability of actin filaments by differential scanning calorimetry
    Beáta Bugyi
    G. Papp
    Sz. Halasi
    B. Visegrády
    Journal of Thermal Analysis and Calorimetry, 2005, 82 : 275 - 279
12345