Induction of apoptosis in leukemia cell lines by Linum persicum and Euphorbia cheiradenia

被引：0
作者：
Zahra Amirghofran
Masoud Bahmani
Abbas Azadmehr
Katayoun Javidnia
机构：
[1] Shiraz University of Medical Science,Immunology Department, Medical School
[2] Shiraz University of Medical Science,Medicinal & Natural Products Chemistry Research Center
来源：
Journal of Cancer Research and Clinical Oncology | 2006年 / 132卷
关键词：
Apoptosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Purpose: In the present study two medicinal herbs Linum persicum and Euphorbia cheiradenia that are native to Iran were tested for their possible anticancer effect and induction of apoptosis on human tumor cell lines including leukemia cell lines. Methods: The effect of methanolic extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. K562 and Jurkat cell lines treated with the extracts were analyzed for the induction of apoptosis by flow cytometry using propidium iodide staining. DNA fragmentation analysis was performed. Results: Various concentrations of L. persicum and E. cheiradenia showed inhibitory effects on different cell lines. Two leukemic lines including K562 and Jurkat were the most sensitive cells for L. persicum with IC50 of 0.1 and 10 μg/ml, respectively. In the cultures of tumor cell lines treated with E. cheiradenia, the main inhibitory effects was for Jurkat cells with IC50 of 12.5 μg/ml. Results indicated a dose-dependent accumulation of cells in the sub-G1 phase. Study of internucleosomal DNA fragmentation showed a typical DNA laddering in agarose gels for both extracts. Conclusion: The present study showed cytotoxic activity of both herbs on tumor cell lines and suggests that this effect may in part be due to the induction of apoptosis in leukemic cells.
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页码：427 / 432
页数：5
相关论文
共 121 条
[1]  
Adam-Klages S(2005)Death receptors and caspases: role in lymphocyte proliferation, cell death, and autoimmunity Immunol Res 33 149-166
[2]  
Adam D(2000)Podophyllotoxin Phytochemistry 54 115-120
[3]  
Janssen O(2003)Modified jatrophane diterpenes as modulators of multidrug resistance from Bioorg Med Chem 11 5221-5227
[4]  
Kabelitz D(2004) L J Nat Prod 67 269-272
[5]  
Canel C(2004)Antitumor activity of bruceantin: an old drug with new promise Mol Cancer Ther 3 1651-1658
[6]  
Moraes RM(1994)Ingenol 3-angelate induces dual modes of cell death and differentially regulates tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in melanoma cells Nucleic Acids Res 22 5506-5507
[7]  
Dayan FE(2002)A rapid and simple method for the isolation of apoptotic DNA fragments J Med Chem 45 2425-2431
[8]  
Ferreira D(2004)Discovery and biological evaluation of a new family of potent modulators of multidrug resistance: reversal of multidrug resistance of mouse lymphoma cells by new natural jatrophane diterpenoids isolated from Euphorbia species J Pharm Biomed Anal 35 441-447
[9]  
Corea G(1976)Comparison of different extraction methods for the determination of podophyllotoxin and 6-methoxypodophyllotoxin in Linum species Science 191 571-572
[10]  
Fattorusso E(2002)Antileukemic principles isolated from Euphorbiaceae plants Zhong Yao Cai 25 135-139
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