High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat

被引：0
|
作者：
Beom Soo Kim
Seung Chul Lee
Sang Yup Lee
Yong Keun Chang
Ho Nam Chang
机构：
[1] Chungbuk National University,Department of Chemical Engineering
来源：
Bioprocess and Biosystems Engineering | 2004年 / 26卷
关键词：
Fed-batch; Exponential feeding; pH-stat;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new feeding strategy in fed-batch culture, exponential feeding combined with pH-stat is suggested to avoid the accumulation of substrate in culture broth. Exponential feeding was stopped whenever a predetermined amount of limiting substrate was supplied and then pH change was observed. When pH rose above an upper limit due to the depletion of substrate, feeding was restarted. With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h−1.
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页码：147 / 150
页数：3
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