Overexpression of PIP5KL1 suppresses cell proliferation and migration in human gastric cancer cells

被引：0
作者：
Lan Shi
Mei Zhao
Qing Luo
Yi-Ming Ma
Jia-Ling Zhong
Xing-Hua Yuan
Chang-Zhi Huang
机构：
[1] Chinese Academy of Medical Sciences and Peking Union Medical College,State Key Laboratory of Molecular Oncology, Cancer Institute and Cancer Hospital
来源：
Molecular Biology Reports | 2010年 / 37卷
关键词：
PIP5KL1; Proliferation; Migration; AKT1; Gastric cancer cell;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Phosphatidylinositol-4-phosphate 5-kinase-like 1 (PIP5KL1), the forth member of phosphatidylinositol-4-phosphate 5-kinases (PIPKs) type I, acts as a scaffold for localization and activation of PIPKs, which mediates numerous cellular processes. However, the role of PIP5KL1 in the development of human cancer is still lacking. We therefore examined the expression of PIP5KL1 in human normal and cancer tissues by tissue microarrays (TMAs). Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence imaging analysis were used to testify the mRNA and protein levels of PIP5KL1 in human gastric cancer cell line (BGC823). The cell proliferation was investigated with 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. Both wound healing and transwell migration assay were performed to study the cell migration. The phosphorylation of v-akt murine thymoma viral oncogene homolog 1 (AKT1) was determined by western immunoblot analysis. Immunostaining of gastric cancer tissue microarrays revealed a negative correlation between PIP5KL1 overexpression and gastric cancer in situ. Transient transfection PIP5KL1 induced a significant increase expression at both transcriptional and translational levels and consequent robust inhibition of proliferation (P < 0.05) and migration (P < 0.05) of BGC823 cells. Overexpression of PIP5KL1 markedly inhibited (P < 0.05) serum-induced phosphorylation of AKT1. Taken together, these studies indicate a functional negative correlation between elevated levels of PIP5KL1 and the development of human gastric cancer, suggesting that PIP5KL1 overexpression may suppress gastric cancer formation.
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页码：2189 / 2198
页数：9
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