Genetic engineering in organoids

被引：0
作者：
Isaree Teriyapirom
Andreia S. Batista-Rocha
Bon-Kyoung Koo
机构：
[1] Vienna Biocenter (VBC),Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
来源：
Journal of Molecular Medicine | 2021年 / 99卷
关键词：
3D Organoids; Organoid Genetics; Pluripotent Stem Cells; Adult Stem Cells; Genetic Engineering; CRISPR/Cas9; Disease Modeling;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy.
引用
收藏
页码：555 / 568
页数：13
相关论文
共 873 条
  • [71] Romero DA(2014)Genome-scale CRISPR-Cas9 knockout screening in human cells Science American Association for the Advancement of Science 343 84-99
  • [72] Horvath P(2016)Growth of human breast tissues from patient cells in 3D hydrogel scaffolds Breast Cancer Research BioMed Central Ltd 18 1-110
  • [73] Bartfeld S(2000)Gene therapy: trials and tribulations Nature Reviews Genetics European Association for Cardio-Thoracic Surgery 1 91-1407
  • [74] Batchelder CA(2011)Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro Nature. Nature Publishing Group 470 105-368
  • [75] Bian S(2006)High prevalence of sessile serrated adenomas with BRAF mutations: a prospective study of patients undergoing colonoscopy Gastroenterology. W.B Saunders 131 1400-62
  • [76] Repic M(2013)Differentiated Troy+chief cells act as reserve stem cells to generate all lineages of the stomach epithelium Cell. Elsevier Inc. 155 357-568
  • [77] Guo Z(2011)Self-formation of functional adenohypophysis in three-dimensional culture Nature Nat Publ Group 480 57-409
  • [78] Kavirayani A(2015)Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis Nature Nat Publ Group 526 564-29
  • [79] Burkard T(2014)Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant Nature Protocols Nat Publ Group 9 396-577
  • [80] Bagley JA(2008)Sessile serrated adenoma (SSA) vs. traditional serrated adenoma (TSA) Am J Surg Pathol 32 21-945
3456789101112