Genetic engineering in organoids

被引：0
作者：
Isaree Teriyapirom
Andreia S. Batista-Rocha
Bon-Kyoung Koo
机构：
[1] Vienna Biocenter (VBC),Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
来源：
Journal of Molecular Medicine | 2021年 / 99卷
关键词：
3D Organoids; Organoid Genetics; Pluripotent Stem Cells; Adult Stem Cells; Genetic Engineering; CRISPR/Cas9; Disease Modeling;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy.
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页码：555 / 568
页数：13
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